辽宁省健康人群C群脑膜炎奈瑟菌血清杀菌力检测

Testing of antibodies against Serogroup C Neisseria Meningitidis by Serum Bactericidal Assay in Healthy Population,Liaoning Province

目的采用血清杀菌力试验,监测辽宁省不同年龄组健康人群血清C群脑膜炎奈瑟菌(Neisseria Meningitidis,Nm)杀菌力水平,以评价健康人群对C群Nm的抗体保护水平。方法随机抽取8个年龄组共240份健康人群血清样品,试验菌株为C群Nm疫苗株(C11)和目前我国C群Nm流行株(053442)。结果240份健康人群血清中,48.33%C群Nm抗体阳性(抗体滴度≥1:2)。对疫苗菌株C11的总保护率(抗体滴度≥1:8)为35.83%,其中≤6岁仅为13.33%,7～19岁61.67%,20～39岁46.67%,≥40岁63.33%。≥15岁健康人群血清对C群流行菌株(053442)的杀菌力低于对C11疫苗株,差异有统计学意义。结论辽宁省≤6岁健康人群对C群Nm血清杀菌力水平较低,实施扩大免疫规划后,≤3岁儿童仍需采取措施提高C群Nm抗体水平。

Objective To test the serum antibodies from healthy population by Serum Bactericidal Assay （SBA）, in order to evaluate the level of protective antibodies against serogroup C Neisseria meningitidis in Liaoning province. Methods 240 serum samples were selected from eight age-group randomly. Serogroup C vaccine candidate strain （C11） and the prevail serogroup C strain （053442） were used for SBA. Results 48.33% of 240 serum samples were positive （titer≥ 1：2） to C 11 vaccine strain. Protective rate of SBA was 35.83%（titer≥ 1：8）, in which,≤6 years old were 13.33%, 7-19 years old was 61.67%, 20-39 years old were 46.67% and ≥40 years old were 63.33%. Rate of SBA to 053442 was lower than that to C11 in the group over 15 years old by statistic analysis. Conclusion Population under 6 years old showed lower SBA capacities. With the implemention of Expanded Program on Immunization, children under 3 years old should be considered how to give them meningococcal vaccine in order to improve the titer of antibodies against Neisseria meningitidis serogroup C.