恶性多形性腺瘤及其周围正常组织的FTIR光谱研究

FTIR Spectroscopic Investigation of Normal and Malignant Pleomorphic Adenoma

文章根据医院临床需要,利用PE公司的Spectrum GX傅里叶变换红外光谱仪研究了多组恶性多形性腺瘤及其周围正常组织结构的FTIR光谱。结果发现:恶性多形性腺瘤与其周围正常组织的红外傅里叶光谱在若干波段处有所不同:相对于正常组织,癌变后核酸分子中的磷酸二脂基团的对称伸缩振动谱带、蛋白质的酰胺Ⅰ带的红外光谱都向高波数移动且吸收都得到增强,说明癌变多形性腺瘤的核酸和蛋白质的氢键化程度普遍增高,吸收普遍增强;而膜脂分子中亚甲基的无序性增加,吸收随之增强。就此可将多形性腺瘤癌区通过红外光谱从正常组织中区分出来。

The FTIR （Fourier transform infrared） spectra of malignant pleomorphic adenoma tissues and surrounding normal tissues were investigated using the Spectrum GX FTIR Spectrometer. The results indicate that there were differences in the Fourier transform infrared spectra between the malignant pleomorphic adenoma tissues and the surrounding normal tissues at some bands. （a） Amide I band location related to the protein in milignant pleomorhic adenoma tissues shifted to a higher wave number compared to the normal tissues,and the absorption was increased in tumor tissues. This indicates that the degree of hydrogen-bonding herein increased; （b） The peak position related to PO2-1 group in nucleic acid shifted to a higher wave number after canceration,and the absorption was stronger in tumor tissues compared to the normal tissues; （c） The absorption of band 1 640 cm-1 related to —CH2 group in lipid was stronger but its peak position shifting was disorder.