蟾毒灵血浆蛋白结合率的测定

Determination of plasma protein binding rate of bufalin

目的：建立血浆中蟾毒灵浓度的分析方法，测定蟾毒灵血浆蛋白结合率。方法：采用平衡透析法测定蟾毒灵血浆蛋白结合率，利用HPLC测定血浆中蟾毒灵浓度，测定并比较大鼠和人血浆中蟾毒灵的血浆蛋白结合率。结果：在0．1～1．0mg·L^-1，药物浓度对血浆蛋白结合率无显著影响，蟾毒灵与大鼠和人的血浆蛋白结合率存在显著性差异，蟾毒灵与人血浆蛋白结合率高于与大鼠血浆蛋白结合率。结论：蟾毒灵与血浆蛋白具有较强的结合。

Objective： To study plasma protein binding rate of bufalin. Method： HPLC was employed to determine the concentration of bufalin. Plasma protein binding rate studies were conducted by equilibrium dialysis method. The influence of drug concentration and plasma in different species on plasma protein binding rate were studied. Result： There was no significant difference in the plasma protein binding rates at low, middle and high bufalin concentrations in dilution medium. The protein binding rate of bufalin in hmnan plasma was higher than in the rat plasma. Conclusion： Bufalin has higher protein binding extent with both rat plasma and human plasma.