膀胱移行细胞癌组织与正常组织中VEGF_(165)b的差异表达及其意义

The differential expression of VEGF_(165)b in the transitional cell carcinoma of the bladder and normal bladder tissues and its clinical significance

目的:初步探讨VEGF165b与膀胱移行细胞癌(Transitional cell carcinoma of the bladder,TCCB)发生发展的关系。方法:用S-P免疫组化法检测38例TCCB组织和36例正常膀胱组织中VEGF165b蛋白表达;以RT-PCR法检测55例TCCB组织和43例正常膀胱组织中VEGF165bmRNA表达。结果:正常膀胱组织中VEGF165b蛋白表达率为97.22%(35/36),TCCB组织中VEGF165b蛋白表达率为21.05%(8/38),表达差异有显著性(P<0.05);正常膀胱组织中VEGF165bmRNA阳性表达率为95.35%(41/43),明显高于TCCB组织中VEGF165bmRNA阳性表达率18.18%(10/55)(P<0.05);TCCB中VEGF165b蛋白和mRNA的表达与TCCB不同分期、分级间的表达差异具有统计学意义(P<0.05)。结论:VEGF165b在TCCB组织中的表达明显低于正常膀胱组织中的表达,这种表达差异可能与VEGF165b抑制膀胱癌的发生、发展作用有关。

Objective：To investigate the differential expression of VEGF165b in the transitional cell carcinoma of the bladder （TCCB） and normal bladder tissues and its role in the development of TCCB. Methods： S-P immunohistochemistry was used to detect the expression of VEGF165b protein in 38 specimens of paraffin-embedded TCCB tissues and 36 specimens of paraffin-embedded normal bladder tissues. RT-PCR was performed to detect the expression of VEGF165b mRNA in 55 specimens of fresh TCCB tissues and 43 specimens of fresh normal bladder tissues. Results： Among 36 of normal bladder tissues, 35 specimens had positive expression of VEGF165b protein, with the positive rate of 97.22% （35/36） that was significantly higher than 21.05% （8/38） （P〈0.05） in TCCB tissues （P〈0.05）. The positive rate of VEGF165b mRNA in normal bladder tissues was 95.35%（41/43）, while that of TCCB tissues was only 18.18%（10/55）. The positive expression rate of VEGF165b mRNA in normal bladder tissues was significantly higher than that in TCCB tissues（P〈0.05）. The expression of VEGF165b was significantly related to clinical stage and pathological grade in TCCB tissues（P〈0.05）. Conclusions： VEGF165b might play a rolein inhibiting development of TCCB.