壬基酚对仔代脑组织AchE和ChAT活性的影响

Effects of nonylphenol on the activity of AchE and ChAT in brian of filial rat

目的:研究母体暴露壬基酚对仔鼠学习记忆及脑组织AchE和ChAT活性的影响。方法:母鼠孕9～15d灌胃壬基酚(0、50、100、200mg/kg),用Morris水迷宫法观察壬基酚对仔鼠学习记忆的影响;检测F1仔鼠垂体和海马组织乙酰胆碱脂酶(AchE)和胆碱乙酰转移酶(ChAT)的活性。结果:与阴性对照组比较,NP200mg/kg组仔鼠水迷宫中逃避潜伏期延长,脑组织ChAT活性明显下降、AchE活性上升(P<0.05)。结论:壬基酚可透过胎盘屏障影响仔鼠学习记忆功能,机理可能与影响胆碱能神经系统功能有关。

Objective：To investigate the effects on the activity of acetylcholinesterase （AchE） and choline acetylase（ChAT） in filial rat’s brian and learning and memory capacity after the mother-rat is exposed to nonylphenol. Methods：At the first day of the pregnancy,the rats were divided into four groups,which were orally administered with NP at doses of 0,50,100 and 200 mg/kg,respectively,on gestational days 9～15. The offspring rats’s Brain tissues of each groups were dissected for AchE and ChAT activity assay and cognitive function was tested by Morris water maze. Result：Morris water maze test results showed the escape latency was longer in the groups of NP 200 mg/kg than in control group（P〈0.05）.The AchE activity decreased while the activity of ChAT increased in the NP 200 mg/kg group, the differences mentioned above were statistical significant compared to control group,with dose-effect relationship in some degree, （P〈0.05）.Conclusion：Exposures to NP during gestation might influenced the activityof AchE and ChAT in brian of F1 rats .