两株转移能力不同的小鼠腹水型肝癌(H_(22))某些生物学特性的比较研究

Comparative Study of Some Biological Characteristics Between Two Murine Ascitcs Hepatocarcinoma Strains (H_(22) and F_(20))with Different Metastatic Potential

从肿瘤移植成活率、淋巴道转移率、细胞形态学、染色体组成、细胞电泳等方面比较了小鼠腹水型肝癌在两种品系小鼠(H_(22)F_0、H_(22)/F_(20))中的差别。形态学结果提示F_0和F_(20)是来源于上皮组织、分化较低的恶性肿瘤。肿瘤移植成活率及淋巴道转移率的比较表明F_(20)瘤株均高于F_0。染色体分析证明,二者在干系细胞的众数及标记染色体的数目及形态均有差别,细胞电泳率的结果显示F_(20)瘤株慢于F_0。以上结果初步证明两种瘤株在采些生物学上存在差别。

Tumour transplantation rate, lymphactic metastic rate, cell morphololgy, chromosomes and cell electroporesis were studied in 2 murine ascites hepatocarcinoma (H_(22) and F_(20))to compare the differences between 2 different strains of mice. The observation of morphology suggests that H_(22) and F_(20) tumours stemmed from epithelial tissue, were poorly differentiated malignant tumours. Comparision of the tumour transplantation rate and lymphatic metastaic rate showed that those of F_(20) were significantly higher than H_(22). Analysis of chromosmes revealed that there were not only differences in chromosome mode of the stem-line but also in number and morphology of marker chromosomes between H_(22) and F_(20). The cell electrophoresis rate of F_(20) was obviously lower than that of H_(22). Above studies proved preliminary that there were some biological difference between H_(22) and F_(20).