自制供肝保存液对大鼠肝分泌细胞间黏附分子-1和一氧化氮影响的比较研究

Comparative study on influence of self-made liver preservation solution to secretion of intercellular adhesion molecule-1 and nitric oxide in rat liver

目的通过与UW液及HC-A液比较,检验自制供肝保存液保存肝脏效果,探讨自制保存液对大鼠肝分泌细胞间黏附分子(ICAM)-1及一氧化氮(NO)的影响。方法无菌条件配制自制供肝保存液;建立大鼠肝脏非循环离体灌注模型(IPRL);根据保存液的不同将大鼠随机分组,检测不同时间点肝灌注流出液中转氨酶含量及肝组织中ICAM-1和NO含量,观察肝组织形态学变化。结果对于丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST),自制液组与UW液组相近,但明显低于HC-A液组(P<0.05);对于ICAM-1和NO,自制液组与UW液组同步升高,两者差异无统计学意义(P>0.05),但与HC-A液比较差异有统计学意义(P<0.05)。同时间点比较自制液组ICAM-1含量较UW液组为高,NO则较低。形态学变化自制液组稍重于UW液组,轻于HC-A组。结论从生化指标和肝脏形态学变化反映出,自制供肝保存液已与世界先进的UW液保存效果相当,明显优于HC-A液。ICAM-1仅在肝细胞损伤时才有表达,其变化程度与酶学指标改变相平行。NO在自制液组较低,反映了自制供肝保存液在维持细胞膜稳定和抑制自由基产生方面具有优势。ICAM-1和NO变化反应肝组织损伤程度,可以做为评价保存液优劣的指标。

Objective To evaluate the effect of self-made liver preservation solution on liver preservation by comparison with UW solution and HC-A solution. To discuss influence of self-made liver preservation solution to secretion of intercellular adhesion molecule （ICAM）-1 and nitric oxide in rat liver. Methods Compound self-made liver preservation solution in aseptic environment. Establish noneirculated isolated perfusion of rat liver. Carry out a randomized grouping experimental study according to the types of solution. Detect aminotransferases content of Krebs-Henseleit＇s perfusate and ICAM-1, NO content of liver tissues in different time points. Results For ALT and AST, the self-made liver preservation solution group are close with UW solution group and lower than HC-A solution group （P〈0.05）. For ICAM-1 and NO, the self-made solution group and UW solution group raise up synchronously, show no statistic significance between both of them（P〉0.05 ）. There are statistic significance compare with HC-A solution group. Self-made solution group is higher than UW solution group, lower than UW solution group at same time point. Self-made solution group is more serious than UW solution and lighter than HC-A solution group on endoscopic changes. Conclusion Biochemical parameters and endoscopic changes reflect that self-made liver preservation solution is overall correspond to UW solution on liver preservation, rather exceed HC-A solution. ICAM-1 only express in the condition of liver cell damage, its level is parallel with enzymological parameters. The lower level of NO content in self-made liver preservation solution reflect that it has superiority in cell membrance stabilization and inhibition of oxygen free radical production. The changes of ICAM-1 and NO reflect the level of liver tissue damage, and can be indicators on evaluation of liver preservation solution.