肠系膜动脉闭塞休克红细胞膜脂质过氧化与膜微粘度关系及硫酸锌的防治作用

A STUDY OF THE RELATIONSHIP BETWEEN LIPID PEROXIDES OF THE ERYTHROCYTE MEMBRANE AND MEMBRANE FLUIDITY, AND THE PROTECTIVE ACTION OF ZINC SULFATE ON SUPERIOR MESENTERIC ARTERY OCCLUSION

目的：探讨肠系膜动脉闭塞性（ＳＭＡＯ）休克红细胞膜丙二醛（ＭＤＡ）水平及膜流动性变化，观察硫酸锌对其有无防治作用。方法：制备家兔肠系膜上动脉闭塞性休克模型，检测红细胞膜ＭＤＡ含量及膜流动性变化。实验分模型组、硫酸锌防治组和生理盐水（ＮＳ）对照组，并进行比较。结果：ＳＭＡＯ休克模型组红细胞膜ＭＤＡ水平明显高于防治组和ＮＳ对照组（Ｐ＜０．０１），红细胞膜流动性低于防治组和ＮＳ对照组；且模型组再灌注后ＭＤＡ含量与膜微粘度有直线正相关关系；防治组ＭＤＡ和膜流动性与对照组相比无显著性差异（Ｐ＞０．０５）。结论：红细胞膜ＭＤＡ水平是影响膜流动性重要原因，硫酸锌对肠系膜动脉闭塞休克有一定防治作用。红细胞膜脂质过氧化损伤是缺血／再灌注损伤的机制之一。

Objective: To study the relationship between the Malondialdehyde(MDA)level of the erythrocyte membrane and the change in membrane fluidity, and the protective action of Zinc sulfate, in connection with Superior Mesenteric Artery Occlusion(SMAO) Methods: A model of SMAO Shock in rabbits were prepared , and then the changes of MDA and membrane fluidity on the erythrocyte membrane were measured The subjects were divided into the SMAO shock model group, zinc sulfate protdction group, and NS control group Results: In terms of the levels of MDA in the erythrocyte membrane, the SMAO shock model group was significantly higher and in terms of the membrane fluidity it was significantly lower, than the zinc sulfate protedtion and the NS control groups (P <0 01) There was , however, no significant difference between the protection group and the NS control group in regard to the levels of MDA and membrane fluidity After reperfusion in the model group there exists a lineal correlation between the levels of MDA and membrane microviscosity Conclusions: The level of MDA is an important factor deciding membrane fluidity, and zinc sulfate may prevent SMAO shock The lipid peroxidation injury of the erythrocyte membrane is one of the pathogenesis of ischemia reperfusion injury