肺癌组织 p53 基因突变的研究

Mutations of p53 Gene in Lung Cancer

目的：探讨肺癌发生的分子遗传学机理，了解肺癌中ｐ５３基因突变的情况。方法：采取聚合酶链反应（ＰＣＲ）及聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）结合银染技术，对２０例肺癌组织中ｐ５３基因外显子５～８进行了突变分析。结果：ｐ５３基因突变１２例，占总人数６０％（１２／２０），分布于外显子５～８。其中第７外显子６例，第８外显子４例，第５、６外显子各１例。鳞癌突变率５０％（３／６），腺癌突变率６０％（６／１０），小细胞肺癌突变率７５％（３／４）。结论：ｐ５３基因突变与肺癌的发生发展有关。

Objective: To detect the relation of p53 gene the development of lung cancer.Methods: We used PCRSSCP methods and silver staining technique to analyse the mutations of p53 gene exons 5～8 in 20 cases lung cancer samples.Results: Mutations of p53 were 12 cases mutation positive which yielded a total mutation rate of 60%(12/20).It were in exon 5～8 which were found that there were 6 cases(30%) of exon 7,4 cases(20%) of exon 8,1 cases (5%) of exon 6,and 1 cases (5%) of exon 5.Mutation of p53 gene were detected in 3 of 6(50%) squamous cell carcinomas,6 of 10(60%) adenocarcinomas,3 of 4(75%) small cell lung carcinomas.Conclusion: p53 gene mutation was a common event in lung cancer which plays an important role during lung cancer.