摘要
目的了解浙江省义乌地区病毒性乙型肝炎(乙肝)病毒(HBV)基因型分布特点,为乙肝的流行病学研究、临床诊治提供参考资料。方法用HBV基因分型(B、C基因型)荧光PCR法直接检测,或用HBV基因型特异引物经聚合酶链反应扩增乙肝患者血清HBV,将目的条带切下,经纯化后测序。测序得到的结果用CLUSTALX及Mega软件进行HBV分型分析。结果该地区基因分型结果,其中B型61.76%(63/102)、C型29.41%(30/102),B/C混合型8.82%(9/102);男、女感染者中B型、C型基因型的构成比较,结果显示差异无统计学意义(χ2=0.03,P>0.05)。B/C混合基因型性别间差异有统计学意义(χ2=4.25,P<0.05);对B基因型和C基因型感染者血清HBV-DNA定量检测结果比较,差异无统计学意义(Uc=1.1213,P>0.05),B基因型和C基因型感染者血清中HBeAg的阳性率分别是33.33%、20.00%,差异无统计学意义(χ2=1.75,P>0.05)。结论浙江义乌HBV基因型为B型、C型和B/C混合型,以B型为主,且具有一定的地域特征。
Objective To investigate the genotype distribution of hepatitis B virus (HBV) in Yiwu and provide reference for the epidemiological study and clinical diagnosis/treatment of hepatitis B. Methods Genotype of HBV (genotype B and C) was identified by using real time PCR directly. HBV genome extracted from hepatitis B patient blood serum was amplified by PCR with specified primers, and then target fragments in the agarose were purified and sequenced. The data were analyzed with CLUSTALX and Mega software. Results The analysis on genotype distribution of HBV among 102 hepatitis B patients indicated that genotype B accounted for 61. 76% (63/102), genotype C accounted for 29.41% (30/102) and genotype B/C accounted for 8.82% (9/102). The results indicated that the differences on genotype B and genotype C cases between males and females were not significant statistically (χ^2 = 0.03, P 〉 0.05 ), but the difference on genotype B/C cases between males and females had statistical significance (χ^2 = 4.25, P 〈 0. 05 ). The difference on HBV-DNA quantitive detection between genotype B cases and genotype C cases had no statistical significance ( Uc = 1. 1213 ,P 〉 0.05 ). The positive rates of HBeAg for genotype B and genotype C cases were 33.33% and 20.00% respectively, which had no significant difference statistically (χ^2 = 1.75 ,P 〉0.05). Conclusion Genotype B, genotype C and genotype B/C of HBV were found in Yiwu, but genotype B was predominant. The genotype distribution of HBV in Yiwu had its own regional characteristics.
出处
《疾病监测》
CAS
2009年第10期774-776,共3页
Disease Surveillance