支气管肺泡灌洗液中结核分枝杆菌检测的方法比较

The Comparison of the Methods Detecting Mycobacteriumtuberculosis in Bronchoalvolar Lavage Fluid

目的：用刷片、集菌、培养、聚合酶链反应等四种方法检测支气管肺泡灌洗液（ＢＡＬＦ）中结核分枝杆菌，对其结果进行比较。方法：采用聚合酶链反应（ＰＣＲ）法对４６例肺结核患者及３２例非结核病患者的标本进行检测，并与刷片、集菌、培养法比较。结果：ＰＣＲ的敏感性显著高于刷片、集菌、培养法。结论：支气管肺泡灌洗液ＰＣＲ结果阳性，可作为早期诊断结核病的重要指标。

Objective: The results of dectecting mycobacterium tuberculosis in bronchoalvolar lavage fluid were compared using smear,concentration of bacille,culture and PCR methods.Methods: The samples from 46 patients with tuberculosis,32 patients without tuberculosis were detected using smear,concentrtion of bacille,culture and PCR methods,and the results were compared,Results: The sensitivity of PCR technique was obviously higher than that of others.Conclusion: It may be the important mark in diagnosis early tuberculosis that mycobacterium tuberculosis is detected positivity in BALF.