钙调素拮抗剂对急性肾毒性肾功能衰竭保护作用的研究

CALMODULIN ANTAGONIST ATTENUATES ACUTE RENAL INJURY INDUCED BY GENTAMYCIN

目的：观察急性肾毒性肾衰时钙调素的变化以及钙调素拮抗剂对肾衰的保护作用。方法：用不同剂量钙调素拮抗剂氯丙嗪对庆大霉素肾毒性肾衰动物进行治疗研究。结果：①庆大霉素肾毒性肾衰大鼠肾组织中钙调素活性无改变，氯丙嗪对钙调素有明显抑制作用。②钙调素拮抗剂对庆大霉素肾毒性肾衰有明显保护作用。并能促进损伤肾小管上皮细胞之再生修复。结论：氯丙嗪等钙调素拮抗剂对急性肾衰有明显保护作用。

OBJECTIVE To define the involvement of calmodulin and the effect of calmodulin antagonist treatment on acute nephrotoxic injury in rats. METHODOLOGY Rat model of acute renal injury was established by repeated gentamycin injections. Tissue levels of calmodulin were determined by the measurement of phosphodiesterase activity. Renal histology was examined and the severity of tubular injury was scored. Serum creatinine levels, urine protein, β 2 microglobulin and lysozyme excretion were monitored after acute renal injury. RESULTS There was no difference in tissue levels of calmodulin before and after acute nephrotoxic injury. However, calmodulin antagonist, chloropromazine treatment significantly decreased renal injury as evidenced by the less severe tubular injury, lower serum creatinine levels and less amount of urine β 2 microglobulin and lysozyme excretion in chloropromazine treated rats than in untreated rats. CONCLUSION There may be a beneficial effect of calmodulin antagonist treatment on acute nephrotoxic injury.