东方拟无枝酸菌发酵液小组分的分离鉴定

Isolation and identification on the metabolites from Amycolatopsis orientalis fermentation

本文首次从万古霉素产生菌东方拟无枝酸菌的发酵液中分离纯化出三个微量组分LYV09lx01、LYV09lx02、LYV09lx03。考察了摇瓶装量的影响,确定了适合LYV09lx01、LYV09lx02富集的摇瓶发酵条件。LYV09lx03则通过万古霉素水解来富集提取。经筛选获得一种具有特异性吸附作用的弱极性大孔吸附树脂AB-8,结合中压液相色谱及高效液相色谱技术进一步纯化鉴定得到一新结构的异黄酮苷。

Three minor components named LYV091x01, LYV091x02 and LYV091x03 were for the first time obtained from the Amycolatopsis orientalis fermentation broth. Conditions suitable for producing LYV091x01 and LYV091x02 were determined based on proper loading volume of fermentation flasks. Another minor component LYV091x03 was enriched by means of the hydrolysate of vancomyein. Several adsorptive resins of different types were tested, among which resin AB-8 was chosen for the primary separation, followed by the combination of medium-pressure LC and HPLC preparations. A new isoflavone compound was obtained, which is elucidated as 4H-1-Benzopyran-4-one, 5,7-[ （6-deoxy-L-mannopyranosyl） oxy] -3- （4-hydroxyphenyl）. The physical and chemical properties and the activity in vitro of these compounds are also preliminary studied.