胸膜肺炎放线杆菌地方分离株和参考菌株的生物被膜形成

Biofilm formation by field isolates and reference strains of Actionbacillus pleuroneumoniae

通过玻璃试管和聚苯乙烯微孔板结晶紫染色法分别对胸膜肺炎放线杆菌(APP)的15个血清型国际参考菌株和22个中国地方分离菌株在体外形成生物被膜的能力进行了定性和定量分析。在15个血清型(无14型)的参考菌株中,只有5b、6和11型能在聚苯乙烯微孔板上形成生物被膜;而59%的地方分离株(涵盖13种血清型,无14和15型)在聚苯乙烯微孔板上表现出产生生物被膜的能力。结果表明,大多数血清型的APP能在体外形成生物被膜,尤其在地方分离株中更普遍;与低毒力血清型的菌株相比,高毒力和中等毒力血清型的菌株更倾向于形成生物被膜。

The biofilm-forming ability of 15 reference strains and 22 field isolates of Actinobacillus pleuropneu-moniae was qualitatively and quantitatively analyzed by glass tube and polystyrene microtiter plate assays. Among the 15 reference strains representing 14 serovars （none of serovar 14） ,only serovars 5b,6 and 11 could form biofilms on the polystyrene surface. In contrast, approximately 59% of field isolates including strains representing 13 serovars （none of serovars 14 and 15） exhibited the ability to form biofilms at different levels determined by the polystyrene microtiter plate assay. Our data indicate that most serovars of A. pleuropneumoniae could form biofilms in vitro ,being more prevalent among field isolates. Generally, highly and moderately virulent serovars showed a higher degree of biofilm formation than low virulent serovars.