高铜对雏鸡脑组织一氧化氮含量和一氧化氮合酶活性的影响

Effect of dietary high copper on the NO content and NOS activity in brain tissue of chickens

1日龄艾维茵肉鸡健雏360羽,随机分为6组,分别喂以对照组日粮(10.89 mg/kg Cu)和高铜日粮(100 mg/kg Cu,高铜Ⅰ组;200 mg/kg Cu,高铜Ⅱ组;400 mg/kg Cu,高铜Ⅲ组;600 mg/kg Cu,高铜Ⅳ组;800 mg/kg Cu,高铜Ⅴ组)6周,观察高铜对雏鸡脑组织和血清一氧化氮(NO)含量及一氧化氮合酶(NOS)活性变化的影响,探讨高铜对雏鸡脑组织的损伤机理。高铜Ⅱ、Ⅲ、Ⅳ和V组脑组织和血清NO的含量较对照组显著或极显著升高(P<0.05或P<0.01)。NOS的活性随日粮铜含量的升高而升高,各高铜组脑组织NOS的活性较对照组极显著升高(P<0.01);高铜Ⅱ、Ⅲ、Ⅳ和V组血清NOS的活性较对照组显著或极显著升高(P<0.05或P<0.01)。日粮铜水平在200 mg/kg及以上时,脑组织和血清NO的含量和NOS的活性随日粮铜的含量升高而升高,脑组织因氧化而受损。

The experiment was conducted to evaluate the effect of dietary high copper on the nitrogen monoxidum （NO）content and nitric oxide synthase （NOS） activity in brain tissue and serum of chickens by the biochemical methods. 360 one-day-old Avian chickens were randomly divided into six groups, and fed on diets as follows：controls（10.89 mg/kg Cu） and high copper （100 mg/kg Cu,high copper group I ;200 mg/kg Cu,high copper group Ⅱ;400 mg/kg Cu,high copper group Ⅲ ;600 mg/kg Cu,high copper group Ⅳ;800 mg/kg Cu,high copper group V） for six weeks. The NO content and NOS activity in brain tissue were much higher in high copper groups Ⅱ, Ⅲ, Ⅳ and V than those of control group （P〈0.05 or P〈0.01）. At the same time,changes of the serum NO content and NOS activity were consistent with those of the brain tissue. These results showed that the NO content and NOS activity were greatly increased by the dietary copper over 200 mg/kg.