昆布多糖对哮喘小鼠治疗作用的研究

Therapeutic effects of Okam on asthmatic mice

目的探讨昆布多糖对哮喘小鼠的治疗作用及机制。方法以卵清蛋白致敏建立哮喘小鼠模型,分别应用高、低剂量昆布多糖,对哮喘小鼠干预4周,观察肺组织病理变化,免疫组化测定肺组织中MMP-9、TIMP-1。结果①试验组喘息症状及肺病理的炎症表现较模型组明显减轻;②试验组肺组织中MMP-9、TIMP-1、MMP-9/TIMP-1较模型组均降低,差异有显著性。结论海洋药物昆布多糖对哮喘小鼠有-定的治疗作用,其机制可能与下调肺组织内MMP-9、TIMP-1的含量及调节MMP-9/TIMP-1的失衡有关。昆布多糖可能开发成为治疗哮喘的一种新药物。

Objective To investigate the therapeutic effects and mechanism of Okam on asthmatic mice. Methods The mice model of asthma was established with the egg albumen sensitization, and were treated with low and large doses of Okam for four weeks. The pathological changes of lung tissues were observed. Immunohistochemistry was used to determine the contents of matrix metalloproteinases-9 （MMP 9） and tissue inhibitor Metalloproteinase-1 （TIMP-1） in the lung tissues. Results Gasp and inflammation in the lung tissues were significantly relieved in the test groups than that in the model group. MMP-9, TIMP-1 and MMP- 9/TIMP-1 ratios were significantly lowered in the test group than that in the model group. Conclusion The marine drug of Okam shows therapeutic effects on mice with asthma, which may be the result of reduced contents of MMP-9 and TIMP-1 in the lung tissues and the correction of the imbalanced MMP-9/TIMP-1 ratio. Okam is likely to be a new choice for the treatment of asthma.