正交实验法优选药用红树植物老鼠簕总黄酮提取工艺

Study on optimum technology for extracting total flavonoids from Acanthus illicifolius by orthogonal design

目的 以老鼠勒为对象，优选出两种黄酮提取工艺中的最佳提取条件。方法利用L9（3^4）正交设计法优选药用红树植物老鼠勒总黄酮提取工艺，以老鼠蕲总黄酮含量为指标，分别对索氏提取中的乙醇浓度、提取温度、提取时间和超声提取法中提取时间、乙醇浓度、液料比等因素进行考查，优选老鼠勃总黄酮的最佳提取条件。结果索氏提取法中，提取温度对总黄酮提取率影响最大，最佳提取备件为60％乙醇提取液，在85℃下提取2．5h。超声提取法中，乙醇浓度对提取率的影响最大，提取最佳条件为50％乙醇提取液，液料比为40：1，浸提50min，提取2次。结论超声提取方法优于索氏提取法，总黄酮提取量可达3．82％。

Objective To study the optimum conditions for extracting total flavonoids from Acanthus illicifolius through the two extraction processes. Methods An orthogonal test of L9 （3^4 ）was designed to select optimum operation conditions for extracting total flavonoids from Acanthus illicifolius through Soxhelt and ultrasonic extraction process. According to the output of total flavonoids under the different extraction conditions, which were selected as ethanol contents, extraction temperatures and extraction time for Soxhelt extraction process, and extraction time, ethanol contents and ratio of liquid to material for ultrasonic extraction process. Results The optimum conditions were established as follows, ethanol content 60%, extraction temperature 85℃ and extraction time 2.5h in Soxhlet extraction process; Ethanol content 50%, extraction time 50min, ratio of liquid to material 40 ： 1 in ultrasonic extraction process. The extraction temperature was the main factor on the Soxhlet extraction process, and the ethanol content on the ultrasonic extraction process. Conclusion The technology of ultrasonic extraction is better than that of Soxhlet extraction to extract the total flavonoids from Acanthus illicifolius. The max output of total flavonoid from Acanthus illicifolius can reach to 3.82%.