海萝多糖对肝癌H_(22)荷瘤小鼠的抑瘤作用研究

Antitumor effects of the polysaccharides from Gloiopeltis furcata on H_(22) tumor bearing mice

目的研究海萝多糖(GFP)对H_(22)移植性肿瘤的抑瘤作用并探讨其抗肿瘤机理。方法建立H_(22)实体肝癌肿瘤模型,以海萝多糖连续灌胃治疗,观察海萝多糖的抑瘤作用,并测定脾脏、胸腺和肝脏等器官的器官系数,分析血清抗H_(22)抗体水平和谷丙转氨酶(GPT)及肝组织谷丙转氨酶(GPT)、谷草转氨酶(GOT)、丙二醛(MDA)水平以及超氧化物歧化酶(SOD)活性等生理生化指标。结果海萝多糖200和400 mg·kg^(-1)·d^(-1)灌胃7d,使给药组抑瘤率分别达到35.64%(P<0.01)和36.63%(P<0.01);其抗肿瘤的机理可能是通过增强机体的特异性免疫、增加血清抗肿瘤抗体水平和增强机体清除自由基的能力来实现;同时还发现海萝多糖具有保护荷瘤小鼠肝脏的作用。结论海萝多糖具有一定的抗移植性小鼠H_(22)肝肿瘤作用。

Objective To study the antitumor effect and the mechanism of the polysaccharide from Gloipeltis furcata on H22, tumor bearing mice. Methods The polysaccharides from Gloipeltis furcata were administered by oral route in mice bearing H22 tumor. The treatments lasted for 7 days. The inhibition rate against H22 tumor and the indices of thymus, spleen and liver were measured. In addition, the levels of antibodies against H22 tumor and GPT in serum, and GPT,GOT,MDA and SOD in liver were measured using commercially available kits. Results The administration of the polysaccharides from Gloipeltis furcata （200 mg·kg^-1·d^-1and 400 mg·kg^-1·d^-1） for 7 days, the inhibition rates of H2z tumor were 35. 64% （P〈0.01）and 36.63%（P〈0.01）, respectively. Its mechanism of antitumor effects maybe enhance the specific immunity and raised the level of antibodies against tumor, and the ability of the free radical scavenging. At the same time, the liver could be protected by the polysaccharide of Gloipeltis furcata in mice bearing H22. tumor. Conclusion The present investigation showed that the polysaccharides from Gloipeltis furcata possess antitumor effect.