湖北海棠种内遗传变异的SRAP分析

The intraspecific variation of Malus hupehensis analyzed by SRAP

应用12对引物对33份湖北海棠样本进行了SRAP分析,每对引物产生4～13条谱带,多态性条带占总带数的91.225%;33个基因型的相似系数在0.5104～0.9271,表明湖北海棠种下具有较丰富的遗传多样性。应用UPGMA法聚类分析表明,在L=0.69时,33份供试材料分为5个组,在L=0.63时,分为3个组。主坐标分析显示,33种供试材料分为5个组,除一份材料外,其余材料分组与聚类分析L=0.69时分组情况相同。在聚类的同一组里,有不同来源地的湖北海棠样品,而在同一来源地的湖北海棠又有的不能聚在同一组,主坐标分析亦获得基本相同的结果,表明湖北海棠的遗传多样性与分布地域间没有对应关系,平邑甜茶或起源于神农架地区。部分材料相似系数较大,有几对甚至难以区分,而兴山35号却与其他类型差别明显大。

Total of 33 accessions of Malus hupehensis（Pamp.） Rehd collected from Hubei Fruit and Tea Research Institute were studied by SRAP-PCR.Twelve out of 36 pairs of primer combinations were selected to amplify the genotypes,with amplification size between 300 bp and 2 000 bp.Each primer pair produced 4 to 13 bands,and polymorphic bands accounted for 91.225%.However,it is difficult to distinguish a given species based on banding pattern from one pair of primers.Similarity coefficient of 33 genotypes ranged from 0.510 4 to 0.927 1.UPGMA analysis indicated that 33 genotypes were categorized into 5 groups at L=0.69,and they were grouped into 3 at L=0.63.According to principal coordinates analysis,33 genotypes were also categorized into 5 groups,same as that from UPGMA analysis except one genotype.The cluster analysis showed that genotypes from different origins were clustered in the same group,and that genotypes from the same origin were divided to different groups.Genetic diversity of Malus hupehensis（Pamp.） Rehd.has no corresponding relation to their origin.The simi-larity coefficient of some genotypes was relatively high while some groups were difficult to distinguish;but Xingshan No.35 was much different from others.