摘要
将溶解在含0.05 mol·L^(-1)聚吡咯的pH 7.2磷酸盐缓冲溶液中乙肝表面抗体固定在多壁碳纳米管碳糊电极上,然后浸入牛血清白蛋白(1+99)溶液中制备聚吡咯/乙肝表面抗体/多壁碳纳米管修饰电极。利用免疫夹心反应原理,将溶液中的乙肝表面抗原和标记有辣根过氧化酶的乙肝第二抗体结合在此电极上。在含有邻氨基酚和过氧化氢的底液中,通过电化学反应生成电活性化合物3-氨基吩呃嗪。此化合物在-0.270及-0.320 V(vs. Ag/AgCl)处出现一对明显的氧化还原峰,其峰电流值与乙肝表面抗体质量浓度在0.2～200μg·L^(-1)范围内呈线性关系。方法的检出限(3S/N)为0.06μg·L^(-1)。
PPy/HBsAb/MWCNT electrode was prepared by immobilizing HBsAb （dissolved in PBS of pH 7. 2 containing 0.05 mol · L^-1 of pyrrole） onto the surface of MWCNT carbon paste electrode, which was further sealed with BSA （1 ＋99） solution. The sealed PPy/HBsAb/MWCNT electrode was reacted with HBsAg and horeadish peroxidase labelled antibody to form a sandwish-like complex, which will feact with 2-aminohydroxybenzene in the presence of H2 O2 to give 2-hydroxy-3-aminophenoxazine（HAPXZ in abort）.A pair of significant redox peaks of the electroactive compound, HAPXZ, were observed at the potentials of -0.270V and --0. 320 V （vs. Ag/AgCl）. Values of peak current were found to keep linear relationship with mass concentration of HBsAg in the range of 0. 2-200μg · L^-1. Detection limit （3S/N） of the method found was 0. 06 μg ·L^-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2009年第11期1320-1322,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
浙江省自然科学基金资助项目(Y4080223)
浙江省教育厅资助项目(20071277)
