不同强度的无创性肢体缺血预适应与经典缺血预适应对大鼠心肌保护作用的对比

The comparative research on different intensity of non-invasive limb ischemia preconditioning and classical ischemic preconditioning on rat myocardial protection

目的:对比不同强度的无创性肢体缺血预适应与经典缺血预适应对大鼠心肌的保护作用。方法:将健康成年雄性wistar大鼠40只,随机平分为4组:经典缺血预适应组(A组)、单下肢远程缺血预适应组(B组)、双下肢远程缺血预适应组(C组)、四肢远程缺血预适应组(D组)。测定血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)。最后取心脏行心脏缺血范围(areaat risk,AAR)、梗死范围(infarcted area,IA)测定。结果:(1)CK和CK-MB:B组及C组的CK和CK-MB活性与A组比均有明显下降(P<0.05),且B组下降最明显。(2)心肌梗死面积(坏死区占左心室重量百分比):与A组比,B组、C组及D组均有明显下降(P<0.05),且B和C组下降最明显,两者无明显差异。结论:无创性肢体缺血预适应比经典缺血预适应对大鼠心肌的保护作用更好,且结扎单下肢对大鼠心肌缺血再灌注的保护作用最强。

Objective To compare different strength of non-invasive limb ischemia preconditioning and classical ischemic preconditioning on the protective effects on rat heart. Methods Healthy adult male wistar rats 40,were randomly divided into four groups： classic preconditioning group （A）, the single-leg remote preconditioning group （B）, the two-leg remote preconditioning group （C）, the limbs remote preconditioning group （D）, Serum creatine kinase （CK）, CK-MB; finally the heart ischemia （AAR）, the scope of infarction （IA） were determinated. Results （1）CK and CK-MB： The levels of CK and CK-MB of B,C and D groups were lower than that of A group. The difference was significant （P 〈 0.05）, especially B group declined obviously. （2） Myocardial infarction area （IA/LV） ： Myocardial infarction area of B, C and D groups were smaller than A group （P 〈 0.05）, especially B and C group declined obviously, but the difference of B and C group was insignificant. Conclusion The protective effect on rat myocardium of non-invasive limb ischemia preconditioning is better than the classic ischemia preconditioning, and the protection is the strongest in the single-leg remote preconditioning group before myocardial infarction.