体外受精移植胚胎培养液中可溶性人白细胞抗原-G的研究

Soluble human leukocyte antigen G expression in embryo culture supernatants after in vitro fertilization and embryo transfer

目的:检测体外受精移植(IVF-ET)胚胎,培养液中胚胎分泌的可溶性人白细胞抗原-G(sHLA-G)水平,探讨sHLA-G与IVF妊娠率的相关性。方法:收集2008年3-6月,唐都医院妇产科生殖中心进行试管婴儿的患者,体外单个培养胚胎的培养液,通过ELISA法检测培养液中sHLA-G的浓度。结果:在41例患者的84份胚胎培养液中,移植至少一个sHLA-G阳性胚胎的妊娠率69%,明显高于全部移植sHLA-G阴性的妊娠率18%(P<0.05)。结论:检测胚胎sHLA-G的表达水平,可以作为辅助形态学评估选择胚胎移植,预测IVF妊娠率。

Objective To measure levels of embryonie secretion of soluble leukocyte antigen G （sHLA-G） from in vitro fertilization and embryo transfer （IVF-ET）, to investigate the correlation between soluble HLA-G and pregnancy rates. Methods Selection patients of in vitro fertilization and embryo transfer （IVF-ET） between March and June 2008 in reproductive center of TangDu hospital, collection the cultured individually embryo culture supernatants, sHLA-G concentration were measured by ELISA assay. Results In the eighty four embryo culture supernatants from forty one patients, when at least one transferred embryos were sHLA-G＋, pregnancy rate were 69%, and only embryos transferred that were negative for sHLA-G had a pregnancy rate of 18%,there was a significant difference（P 〈 0.05）. Conclusion Addition of measures sHLA-G concentration to traditional morphological criteria may be useful to embryo selection, predict pregnancy rate of IVF.