摘要
目的:建立防风通圣丸中连翘苷的含量测定方法。方法:采用高效液相色谱法、色谱柱为Diamonsic18(250mm×4.6mm,5μm),流动相为乙腈-水(25:75),检测波长为277nm。结果:连翘苷在0.025mg~0.400mg范围内线性关系良好,连翘苷的平均加样回收率为99.63%,RSD为0.20%。结论:该方法准确可靠,分离度好,可用于防风通圣丸的质量控制。
Objective : To establish a method to determine the content of forsythin in Fangfengtongsheng pill. Method : HPLC was used. The separation was performed on Diamonsil C18 (250 mm × 4.6 mm,5 μm)with acetonitrile -water( 25:75 ) as a mobil phase the de- tecting wavelength was at 277 nm. Results: Forsythoside showed a good linear relationship in the range of 0. 025 mg - 0. 400 mg ( r = 0. 9996), the average recovery of forsythin was 99.62%. RSD was 0.20%. Conclusion:This method is accurate, also showing good separating degree. It can be used in quality control of Fangfengtongsheng pill.
出处
《中医学报》
CAS
2009年第6期38-39,共2页
Acta Chinese Medicine