HPLC法同时测定阿咖酚胶囊中三种成分的含量

Determination of Three Constituents in Paracetamol Caffeine and Aspirin Capsules by HPLC

目的：建立同时测定阿咖酚胶囊中三种成分（对乙酰氨基酚、咖啡因、阿司匹林）的HPLC法。方法：色谱柱为Phenomenex C8柱（4．6mm×250mm，5μm），流动相为磷酸盐缓冲液（取0．01mol·L^-1磷酸二氢钾溶液，用磷酸调节pH至2．6±0．1）-甲醇（65：35），流速为1．0mL·min^-1，检测波长为229nm，柱温为30℃。结果：对乙酰氨基酚、咖啡因和阿司匹林的进样量分别在0．4084～4．084，0．0938～0．938和0．7476～7．476μg范围内与峰面积呈良好线性关系，r分别为1．0000，1．0000，0．9999（n=6），平均加样回收率分别为100．6％，100．5％和100．3％，RSD分别为0．8％，1．6％和1．2％（n=9）。结论：本方法快速简便，准确可靠，可用于阿咖酚胶囊的质量控制。

Objective：To establish a method for determination of three constituents（ paraeetamol, caffeine and aspirin）in Paraeetamol Caffeine and Aspirin Capsules by HPLC. Method：The analytical column was Phenomenex C18 （4.6mm×250mm,5μm） set at 30 ℃ ;The mobile phase was phosphate buffer （0. 01 mol · L^-1 potassium phosphate monobasic adjust to pH 2. 6± 0. 1 by phosphoric acid）-methanol （65： 35） with flow rate 1.0 mL · min^-1. Detection wavelength was set at 229 nm. Results： The linear ranges of Paracetamol, Caffeine, and Aspirin were 0. 408 4 -4. 084,0. 093 8 -0. 938 and 0. 747 6 -7. 476 mg · L^-1 with relative coefficient r = 1. 000 0,r = 1. 000 0, and r = 0. 999 9 （ n = 6 ） , respectively. The average recoveries of Paracetamol , Caffeine and Aspirin were 100. 6 % （RSD =0. 8% ）, 100. 5% （RSD = 1.6% ）and 100. 3% （RSD =0. 8% ）, respectively. Conclusion：The method is simple , accurate ,and suitable for determination of the three constituents in the Paracetamol,Caffeine and Aspirin Capsules.