高效液相色谱紫外检测法测定人血浆中酮洛芬浓度

Determination of ketoprofen in human plasma by HPLC-UV

目的建立高效液相色谱紫外法测定人血浆中酮洛芬浓度。方法用乙醚提取血浆样品中酮洛芬及酮洛酸（内标）,采用Waters C18色谱柱（5μm,4.6 mm×150 mm）,以甲醇∶水∶冰醋酸∶三乙胺=（48∶52∶0.2∶0.3）为流动相,流速为1.0 ml.min^-1,在268 nm波长下检测。结果酮洛芬和酮洛酸的保留时间分别为10.1 min和5.0 min,线性范围为0.10-10.46 mg.L^-1,最低检测浓度为0.02 mg.L^-1,线性关系良好。高、中、低浓度酮洛芬的准确度在85%-115%之间,批内和批间的变异系数均〈15%,提取回收率大于70%。结论本方法简便、准确、重复性好,适用于人血浆中酮洛芬浓度测定及药代动力学研究。

Aim To establish an HPLC-UV method for determining ketoprofen in human plasma.Methods Ketoprofen and ketorolac（I.S） in human plasma,extracted from human plasma by ether,were analyzed on a waters C18 column（5μm,4.6 mm ×150 mm）.Methanol-water-acetic acid-triethy lamine（48∶52∶0.2∶0.3） was used as isocratic mobile phase at a flow rate of 1.0 ml/min.The wavelength was set at 268 nm.Results The retention times of ketoprofen and Ketorolac were about 10.1 and 5.0 min,respectively.The assay was linear over the concentration range 0.10 - 10.46 mg · L^-1 excluded from endogenous substance effect. The intra-and inter-batch precision over the entire concentration range were less than 15%. The mean extraction recoveries of ketoprofen were higher than 70%. Conclusion A simple,rapid and specific HPLC-UV method was developed to determine ketoprofen in human plasma which is suitable for clinical pharmacokinetic studies.