微生物酶催化法生产丙烯酰胺的研究

STUDY ON PRODUCING ACRYLAMIDE BY MICROBIAL ENZYME CATALYSIS

研究了微生物酶催化法生产丙烯酰胺新工艺。经诱变育种和驯化，菌株Aeromonassp．L401－Ⅱ44的腈水合酶比活性提高到600μmol／（min·mg），发酵培养生产效率提高3．5～5．5倍。制成颗粒状固定化细胞，用该固定化细胞制成填充柱形生物反应器，实现了由丙烯睛转化成丙烯酰胺的连续操作工艺。固定化细胞的酶活性回收率达到70％～100％，不同温度下酶的稳定性明显提高。丙烯酰胺产品无色素，免除了脱色工序。用该AM单体聚合能得到1．5×107以上高分子量聚丙烯酰胺，水溶性良好。

A new process for producing acrylamide by microbial enzyme catalysis has been studied.Under inducing mutation and breeding, the specific activity of nitrile hydrase of strainAeromonas sp. L 401- Ⅱ 44 has increased to 600 μmol/min mg and the production efficiencyo f fermentive culture has been raised by 3. 5~ 5. 5 times as compared with that of parentstrain. The strains are made in granular immobilized cells. The columnar biological reactor isfilled with the cells, thus achieving continuous operation for converting acrylonitrile intoacrylamine. The enzyme stability of immobilzed cells is obviously raised in various temperatures and the residual activity is 70% -100%. There are no pigment in acrylamide products.Molecular weight of polyacrylamide obtained from acrylamide monomer is over 1. 5 × 107. It' swater solubility is excellent.