小鼠血液阻抗频谱特性及其等效电路模型分析

Analysis on Impedance Spectral Characteristic and Equivalent Circuit Model of Mouse Blood

在0.01～100MHz频率范围,使用Agilent 4294A阻抗分析仪测量了小鼠血液细胞交流阻抗,通过Bode图、Nyquist图及Nichols图的数据以及等效电路模型曲线拟合分析,建立了小鼠血液细胞阻抗频谱特性和等效电路模型参数.研究表明:(1)小鼠红细胞阻抗谱的幅模量和相位角具有频率依从性;(2)小鼠血液细胞阻抗频谱具有2个特征频率(FC1=2.8MHz,FC2=4.2MHz);(3)小鼠血液整体细胞膜电容Cm=120F·cm-1,细胞膜电阻率>10MΩcm.

The AC impedances of mouse blood cells are measured with the Agilent 4294A impedance analyzer at frequency range of 0.01～100 MHz,then its spectral characteristic and equivalent circuit model parameters are determined by the Bode plot,the Nyquist plot and the Nichols plot.The results show that：（1）The impedance amplitude and phase angle of mouse blood cell are frequency-dependent;（2）The impedance spectrum of mouse blood cell have two characteristic frequencies（FC1=2.8 MHz,FC2=4.2 MHz）;（3）The whole blood cell membrane capacitance Cm=120 F·cm^-1,and cell membrane resistivity is found to be more than 10 MΩcm.