医院空气中黄曲霉菌含量分析研究

Analysis of Air Concentration of Aspergillus Flavus in Hospital

目的了解医院空气中黄曲霉菌污染程度和规律。方法采用离心式空气微生物采样器和真菌培养的方法,在1年中不同季节分别选择1 d中不同的5个时段,对医院不同环境空气中黄曲霉菌进行采集、培养、计数和分析。结果所有采样点在4个季度均采集到了黄曲霉菌株;6个环境区空气中黄曲霉菌含量不同,以门诊楼最高,平均达1238.9 CFU/m3;其中内科楼各科室空气中黄曲霉菌含量各季节间差异有统计学意义(P<0.05);均数两两比较显示,春、秋季与冬、夏季间差异有统计学意义,而冬季与夏季、春季与秋季差异无统计学意义;外环境各季节间差异无统计学意义(P>0.05);同时内科与外环境空气中黄曲霉菌含量在冬、夏季差异有统计学意义(均P<0.05),春季、秋季差异无统计学意义(均P>0.05)。结论医院环境中黄曲霉菌污染较严重,加强环境控制,预防曲霉菌属感染非常必要。

OBJECTIVE To study the degree and regular of Aspergillns flavus air contamination in hospital. METHODS Some ambient air of different environment in our hospital was taken 5 times in one day of four seasons one year by using centrifugal air sampler, and then cultivated （using eumycete cultural methods）, counted and analyzed. RESULTS A. flavus strain was collected in all sampling points in four seasons. The concentration of Aspergillus flavus in six standort was diverse from each other, and the highest was the out-patient clinic with 1238.9 CFU/m^3. The concentration of Aspergillus flavus in each division of Medical Department was significant difference during four seasons （P〈0.05）. The mean in spring and autumn was different from that of winter and summer. While no significant difference was found between winter and summer or between spring and autumn. Also there was no significant difference in external environment （P〉0. 05）. The difference between internal medicine and external enviroment was significant between the first and the third season（ P= 0. 022, P= 0. 039）, but that of the second and the fouth season was not significant （P= 0. 022, P = 0. 624）. CONCLUSIONS The contamination of Aspergillus flavus in hospital was severe. So it＇ s necessary to prevent the Aspergillus flavus infection and enhance the environmental control of susceptible population.