蝉拟青霉LB菌株的生物学特性

Biological Characteristics of Paecilomyces cicadae LB Strain

本文研究碳源、氮源、温度、湿度、pH值和光照等对蝉拟青霉LB菌株生长、产孢和孢子萌发的影响。结果表明,适合该菌株菌落生长和产孢的最佳碳源是可溶性淀粉和蔗糖,最佳氮源为蛋白胨;菌丝生长和孢子萌发的最适温度范围是25°C^27°C,产生分生孢子的最适温度是25°C;分生孢子萌发所需湿度范围是RH 90%~100%,当RH低于90%时很难萌发;在pH值4~10的范围内该菌能生长和产孢,菌丝生长最适pH为6,产生分生孢子和孢子萌发最适pH范围为6~7;光照处理对该菌产孢有一定的影响;分生孢子的致死条件为55°C 10 min。生物学特性显示,蝉拟青霉LB菌株是一株对营养要求不高、对环境适应能力较强的昆虫病原真菌。

The effect of carbon source, nitrogen source, temperature, moisture, pH and light on the fungal growth, conidia production and conidia germination of Paecilomyces cicadae LB were studied. For the fungal growth and conidia production, the optimum carbon sources were soluble starch and glucose, while the optimum nitrogen source was peptone. For the fungal growth and spore germination of P. cicadae, the optimum temperature was 25℃-27℃, for the conidia production, the optimum temperature was 25℃. Conidia geminated at RH 90%-100%, but did not below 90%. When RH reaches to 100% or the conidia were in water, the germination rate was the highest. The range of pH for the fungal growth, conidia production and conidial germination was 4-10, while the optimum pH for the fungal growth was 6 and 6-7 for the conidia production and conidial germination. The light treatment significantly influenced fungal conidia production. The lethal temperature for the fungal conidia was 55℃ remaining 10 minutes. The present results suggest the isolate LB can adapt to nutrition and environment more widely, and has greater potential as biological control factor against of pest.