PPARγ和NF-κB在肺纤维化中的表达与意义

Expression and role of peroxisome proliferator-activated receptor γ and nuclear factor-κB in pulmonary fibrosis

目的:观察过氧化物酶体增殖物激活受体γ(PPARγ)和核因子-κB(NF-κB)在肺纤维化患者肺组织中的表达情况,探讨其在肺纤维化发病中的作用。方法:采用免疫组织化学方法检测16例肺纤维化患者与10例正常对照肺组织标本中PPARγ、NF-κB的表达情况。结果:肺纤维化组PPARγ的阳性表达记数为0.35±0.08,低于正常对照组的0.42±0.04(P<0.05)。肺纤维化组NF-κB的阳性表达记数为0.51±0.11,高于正常对照组的0.38±0.04(P<0.05)。肺纤维化组的PPARγ和NF-κB的表达呈负相关(P<0.05)。结论:肺组织中PPARγ的表达减弱、NF-κB的表达增强在肺纤维化的发病过程中起作用,为临床治疗肺纤维化提供了新思路。

Objective： To evaluate the expression of peroxisome proliferator-activated receptor γ （PPARγ） and nuclear factor-kB （NF-kB） in lungs in patients with pulmonary fibrosis, and to explore their effect on the pathogenesis of pulmonary fibrosis. Methods： Immunohistochemical technology was performed to investigate the PPARγand NF-kB expression in lung specimens from 16 cases of pulmonary fibrosis and 10 cases of normal controls. Results： The positive score of PPAR3, （0. 35 ± 0. 08 ） in fibrosis group was lower than that in control group （0. 42 ± 0.04, P 〈 0. 05）. The positive score of NF-kB （0. 51 ±0. 11 ） in fibrosis group was higher than that in control group （0. 38 ±0. 04, P 〈0. 05）. There was negative correlation between PPARγ and NF-kB expression in fibrosis group （ P 〈 0. 05 ）. Conclusion： The decreased expression of PPARγ and enhanced expression of NF-kB play a role in the pathogenesis of pulmonary fibrosis, which may provide a new idea for treating this disease.