女贞子多糖对黑色素瘤细胞黏附能力的影响

The adhesion effect of Ligustrum lucidum Ait polysaccharide on melanoma B16BL6 in vitro

目的探讨女贞子多糖对黑色素瘤细胞B16BL6黏附能力的影响。方法观察女贞子多糖对黑色素瘤细胞黏附力的抑制作用,分别将琼脂糖和Matrigel铺于细胞板中,用女贞子多糖作用后,MTT法观察女贞子多糖对B16BL6黏附能力的影响;同时观察了女贞子多糖对黏附分子E-cadherin表达的影响。结果女贞子多糖抑制了黑色素瘤细胞的粘附能力,抑制黑色素瘤粘附分子E-cadherin的表达。结论女贞子多糖具有抑制黑色素肿瘤细胞的黏附,破坏了肿瘤细胞的整体性,利于女贞子多糖的抗肿瘤作用。

Aim To investigate the effects of the poly- saccharides from Ligustrum lucidum Ait（LLP） on melanoma B16BL6 adhesion in vitro. Methods The effect of LLP on melanoma B16BI/5 adhesion in vitro was evaluated by MTT assay on agarose and matrigel. Also the effect of LLP on the expression of adhesion molecule E-cadhesion in melanoma B16BL6 in vitro was studied. Results LLP had an obvious inhibition on melanoma B16BL6 adhesion and decreased the expres-sion of adhesion molecule E-cadhesion in melanoma B16BL6 in vitro. Conclusion LLP can inhibit the adhesion of melanoma B16BE6 cells in vitro and dampen the system of B16BE6 cells, and then be good at the effect of LLP in the of anti-tumor activity.