二甲双胍对多囊卵巢综合征模型大鼠血清抑制素B、激活素A、卵泡抑素水平的影响被引量：5

Effect of metformin on the inhibinB,activinA and follistatin in polycystic ovary model rats

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摘要目的观察二甲双胍对多囊卵巢(polycystic ovary,PCO)模型大鼠血清抑制素B(INHB)、激活素A(ACTA)、卵泡抑素(FS)水平的影响,从卵巢局部细胞因子的角度探讨其对PCOS的治疗作用并分析其可能的作用机制。方法采用胰岛素联合HCG造模法制备PCO大鼠模型,运用放射免疫法测定血清性激素、胰岛素(INS)水平;双抗体夹心酶联免疫吸附法测定INHB、ACTA及FS的水平。结果二甲双胍能够下调PCO大鼠血清睾酮(T)、促黄体生成素(LH)、INS水平;并上调血清ACTA水平,升高血清ACTA/INHB比值;且发现INS与ACTA呈负相关,与INHB呈正相关。结论二甲双胍能够调节卵巢局部细胞因子INHB、ACTA、FS的表达。 Aim To observe the effect of metformin （MF） on inhibinB, activinA and follistatin in rat of polycystic ovary （PCO）, analyzing the possible therapeutic mechanism and therapeutical effect from ovaries local cytokine. Methods The PCO rat models were set by insulin combined with human chorionic gonadotrophin（HCG）. The levels of serum gonadal hormone and insulin（INS） were detected by radioimmunoassay. Using the sandwich ELISA technique the expressed level of serum inhibinB （ INHB ） , activinA （ ACTA ） and fol-listatin （FS） was detected. Results Metformin could decrease the level of testosterone （T） , luteotropic hormone（LH） and INS in rat serum, but it increased the level of ACTA in rat serum and the rate of ACTA/IN- HB. There was negative correlation between INS and ACTA, and positive correlation between INS and IN- HB. Conclusion MF can regulate the expression of ovaries local cytokines such as INHB, ACTA and FS.

作者陈梅刘润侠

机构地区西安交通大学医学院第一附属医院生殖医学科第二附属医院中西医结合科

出处《中国药理学通报》 CAS CSCD 北大核心 2009年第10期1370-1373,共4页 Chinese Pharmacological Bulletin

基金陕西省科技攻关资助项目(No2005K16-G5(2))

关键词二甲双胍多囊卵巢综合征抑制素B 激活素A 卵泡抑素性激素胰岛素 metformin polyeystic ovarian syndrome INHB ACTA FS gonadal hormone insulin

分类号 R-332 [医药卫生] R322.65 [医药卫生—人体解剖和组织胚胎学]

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