结直肠癌患者体内氧化应激状态的评价及产物的分析

Antioxidant status and products of oxidative stress in colorectal cancer

目的:机体的抗氧化能力和氧化应激状态都与大肠癌相关,文中评价结直肠癌(CRC)患者体内的氧化应激水平,研究氧化应激与CRC的关系。方法:对60位研究对象(CRC患者和正常对照各30名)的血清,分别检测蛋白质、脂类、DNA的氧化损伤产物;检测维生素C、维生素E、谷胱甘肽等抗氧化剂及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等抗氧化酶类。结果:CRC患者体内蛋白羰基、晚期蛋白质氧化损伤产物均比对照组有明显升高(P<0.01),而CRC患者体内丙二醛和共轭二烯比对照组明显下降(P<0.01);CRC患者血清中抗氧化酶类的活性、维生素C和维生素E的水平显著低于对照组(P<0.01)。结论:CRC和氧化应激相关,评价CRC患者体内氧化应激状态对预测CRC的发生有指导意义。

Objective：Colorectal cancer is related to antioxidative ability and oxidative stress level.The aim of the study was to evaluate the oxidative stress in colorectal cancer patients and to investigate the relationship between oxidative stress and colorectal cancer.Methods：A total of 60 subjects（30 colorectal cancer patients,30 normal healthy controls） were examined in the study and estimated the protein oxidation,DNA damage,lipid peroxidation and antioxidants-vitamin C,vitamin E,glutathione（GSH） and antioxidative enzymes in serum,respectively.Results：Statistically significantly higher values of protein carbonyl and advanced oxidation protein products（AOPP） were observed in colorectal cancer patients（P〈0.01）.There was a significant increase in serum 8-OHdG in colorectal cancer patients compared to normal healthy controls（P〈0.01）.But the mean serum level of MDA and conjugated diene were lower in colorectal cancer than that in normal healty control（P〈0.01）.There was a significant decreased levels of activity of antioxidative enzymes in colorectal cancer patients compared to normal subjects（P〈0.01）.Serum vitamin C and vitamin E concentrations were significantly reduced in colorectal cancer patients compared to healthy controls（P〈0.01）.Conclusion：Our results indicate that colorectal cancer is associated with oxidative stress.