仿生技术构建小口径人造血管

Experimental Study of the Bionic Technology for the Construction of Small-caliber Vascular Prostheses

目的:研究以改性猪小肠粘膜下层组织(SIS)为支架,利用仿生技术构建小口径人造血管的可行性。方法:自犬隐动脉分离出血管内皮细胞和平滑肌细胞,与胶原蛋白凝胶均匀混合,分别种植于改性SIS膜表面,制成3 mm仿生三层人造血管为实验组;制成单层人造血管为对照组,分别植入修复15例犬双侧股动脉缺损,术后进行彩超、组织学检测和电镜检测鉴定。结果:植入12周,14个仿生人造血管保持通畅,通畅率93.3%,有血管样生物结构形成,管腔内壁有完整的内皮覆盖,管壁中层见大量平滑肌细胞;对照组通畅率60%,有少许内皮细胞覆盖。结论:仿生小口径人造血管具有良好的血液相容性,并能在体内保持良好通畅性,修复动脉缺损效果满意。

Objective ： study the feasibility of using the bionic technology to construct small-caliber vascular prostheses with modified SIS . Methods： The vascular endothelial cells and smooth muscle cells were separated from canine saphenous artery, the cells blended with collagen gel, which were planted respectively on the SIS films, these films were rolled into the biologic three-layer prostheses around a 3mm diameter polyethylene tube ; one-layer prostheses without these cells and collagen gel served as control. These 2 types of prostheses were implanted into the defect of bilateral canine femoral by anastomosis in 15 dogs. doppler colour ultrasonic, histology detection and electron microscope examination were done postoperatively. Results： By 12 weeks postoperatively, 14 biologic vascular prostheses had kept well patency, the patency rate being 93. 3%, the biologic structure like blood vessels had formed, the inner surface of the vessels had been covered with full endothelial ceils, a lot of smooth muscle cells had been found in the media of vascular walls in regular line; the patency rate in control group was 60.0%, the endothelial cell coverage was incomplete. Conclusions ： The bionic vascular prostheses showed potent blood compatibility, which could keep long-term patency in vivo. Curative effect of repairing the small-caliber vessel defect was well satisfactory.