生长素、乙烯和一氧化氮对拟南芥下胚轴插条形成不定根的调节

Regulation of Auxin,Ethylene and Nitric Oxide on Adventitious Rooting in Arabidopsis Hypocotyl Cuttings

研究生长素、乙烯和一氧化氮(NO)对拟南芥下胚轴插条形成不定根的调节,以及生长素和乙烯信号转导成员在IAA促进不定根形成中的作用的结果表明:拟南芥切条以IAA和硝普钠(NO供体)单独处理7d后的不定根形成均受到促进,其中以50μmol·L-1IAA和1μmol·L-1SNP的促进作用为最强,乙烯的促进作用不明显;生长素运输和信号转导以及乙烯信号转导相关突变体对IAA促进生根作用的敏感性比野生型有所下降,特别是IAA14功能获得型的突变体。IAA和NO在促进不定根形成中有协同效应。

Arabidopsis hypocotyl cuttings were employed to study effects of auxin, ethylene and nitric oxide （NO） on adventitious rooting. The functions of auxin and ethylene signaling players cooperated with indole acetic acid （IAA） in adventitious rooting were also investigated. The results showed that treatment with either IAA or sodium nitroprusside （SNP, donor of NO） on Arabidopsis cuttings for 7 days could stimulate adventitious root formation, and the optimal concentration is 50 μmol·L^-1 and 1 μmol·L^-1, respectively; while ethylene played little role in rooting. Compared with wild type, sensitivity of auxin transportation and signal transduction and ethylene signaling-related mutants to stimulatory effect of IAA on rooting was reduced, especially gain-offunction of mutated IAA14 （SLR）. NO had synergistic role with IAA in adventitious rooting.