基质金属蛋白酶-26在多种肿瘤组织及平滑肌细胞中的表达(英文)

Expression of matrix metalloproteinase-26 in multiple human cancer tissues and smooth muscle cells

背景与目的:基质金属蛋白酶(matrix metalloproteinases,MMPs)在多种癌组织中表达升高。MMP-26在前列腺癌和乳腺癌组织中高表达,通过裂解纤维连接蛋白和Ⅳ型胶原蛋白,并激活酶原型MMP-9(一种强效明胶酶)来促进人前列腺癌细胞的转移。本研究旨在探索MMP-26在多种人类肿瘤组织中的表达谱。方法:以免疫组化法及多重组织芯片检测MMP-26的蛋白表达;以RT-PCR检测冠状动脉平滑肌细胞中的MMP-26mRNA表达。结果:与相应正常组织相比,MMP-26在乳腺癌、结肠癌、肺癌、脑肿瘤、头颈部肿瘤、前列腺癌和黑色素瘤组织中的表达显著升高(P<0.05),但在肾癌、卵巢癌和非霍奇金瘤组织中未见明显升高(P>0.05)。胃癌、直肠癌、甲状腺癌、气管癌和胰腺癌中也检出MMP-26表达。MMP-26表达于前列腺及相应血管的平滑肌细胞。冠状动脉平滑肌细胞中也检出MMP-26mRNA表达。结论:MMP-26表达可能与多种人类肿瘤有关,可作为这些肿瘤早期诊断的分子指标。MMP-26也可能参与人前列腺和心血管系统的平滑肌功能。

Background and Objective. Elevated expression of matrix metalloproteinases （MMPs） has been found in multiple carcinoma tissues. MMP-26 is highly expressed in prostate and breast cancer tissues, and promotes the invasion of human prostate cancer cells not only through the cleavage of fibronectin and type IV collagen but also by the activation of pro-MMP-9, a powerful gelatinase. This study was to present a comprehensive protein expression profile of MMP-26 in multiple human cancer tissues. Methods.. The protein expression pattern of MMP-26 was examined using immunohistochemistry and multiple-tissue microarray. MMP-26 mRNA expression in coronary artery smooth muscle cells was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results： The expression of MMP-26 in breast, colon, lung, brain, head and neck, prostate cancer, and melanoma tissues was significantly elevated when compared with parallel normal tissues （P〈0.05）, while not significantly elevated in kidney cancer,ovarian cancer, and non-Hodgkin＇s lymphoma （P〉0.05）. MMP-26 was also detected to express in gastric, rectal, thyroid, esophageal, and pancreatic cancers. MMP-26 protein was expressed in smooth muscle cells of the prostate and associated blood vessels. MMP-26 mRNA was also detected to express in human coronary artery smooth muscle cells. Conclusions：MMP-26 expression may be associated with multiple human carcinomas, and it may serve as a molecular marker for the early diagnosis of these carcinomas. MMP-26 may also contribute to smooth muscle function in the human prostate and cardiovascular system.