枳实提取物柚皮苷大鼠在体肠吸收动力学

Study of naringin in extract from Citrus aurantium on rat intestinal absorption kinetics in situ

目的考察柚皮苷大鼠的在体肠吸收动力学性质。方法采用大鼠在体单向灌流法,利用HPLC法测定柚皮苷的含量,分别研究吸收部位、药物浓度、灌流速度对柚皮苷吸收的影响。结果各肠段间药物的吸收速率常数Ka和表观吸收系数Papp比较均无明显差异(P>0.05);柚皮苷浓度在5.63～30.2mg·L-1范围内,吸收速率常数Ka和表观吸收系数Papp比较均无明显差异(P>0.05);随着灌流速度的增加,吸收速率常数Ka和表观吸收系数Papp均明显增加。结论柚皮苷在全肠道均有吸收,且无特定吸收部位;吸收无高浓度饱和现象,提示柚皮苷吸收主要为被动扩散机制。

AIM To study the rat intestinal absorption kinetics of naringin in s itu. METHODS Singlepass perfusion model was used for rats in situ, the naringin content was measured by HPLC and the absorption sites, perfusate concentration with flow speed were investigated in order to reveal their influences on the absorption of naringin. RESULTS The statistics showed that the Ka and the P,w values of the duodenum, jejunum, ileum and colon had no significant differences （P 〉 0.05）. The concentrations of naringin from 5.63 - 30.2 mg ＂L-1 demonstrated no distinctive effect on the Ka and the Papp （P 〉 0.05） , Ka and Papp increased significantly with the up raising of perfusion speed. CONCLUSION Naringin could be absorbed in all intestine tract of rats without special absorption sites and no highest saturated Concentration. Passive diffusion mechanism would be suggested as the major factor for the absorption of naringin.