摘要
目的:探明salusin-β——一种新发现的心血管调节肽是否具有抗心肌缺血/再灌损伤(RMMI)的作用。方法:以离体灌流大鼠的心脏RMMI为模型,分为对照组和salusin-β预处理组,观察各组大鼠心脏缺血/再灌注(I/R)后的心率(HR)、冠脉流量(CF)和心功能[左室收缩压(LVSP)、左室舒张末压(LVEDP)和左室内压上升、下降速率(±dp/dt)]的变化。结果:以10-8mol/L的salusin-β预处理,可以改善心脏I/R后CF、LVDP、LVEDP、+dp/dt和-dp/dt等指标。10-8mol/L salusin-β组复灌30 min时,CF、LVDP、+dp/dt和-dp/dt的恢复率,分别是停灌前的(52±5)%、(79±8)%、(70±8)%和(69±10)%,显著高于对照组的(45±6)%、(68±10)%、(58±11)%和(57±8)%(P<0.01,n=8)。此外,10-8mol/L salusin-β组复灌30 min时,LVEDP为(28.25±7.62)mmHg,显著低于对照组的(52.52±11.27)mmHg。结论:上述结果表明,salusin-β具有抗心肌RMMI、改善I/R后心脏功能的作用。
AIM: To investigate whether salusin-β, a new regulatory peptide relevant to the cardiovascular system, can alleviate heart ischemia-reperfusion injury. METHODS: The effects of salusin-β on heart rate ( HR), coronary flow (CF) and heart function ( LVSP, LVEDP and ±dp/dt) were observed in isolated rat hearts after isehemia-reperfusion. RESULTS: Treatment with 10^-8 mol/L salusin-β significantly improved the post-ischemie CF, LVDP, LVEDP and ± dp/dt compared with those of the untreated hearts. The recovery of CF, LVDP, + dp/dt and dp/dt after reperfusion for 30 min was, respectively, (52±5)%, (79±8)%, (70±8)% and (69±10)% vs. (45±6)%, (68±10)%, (58±11)% and (57±8)% in control group (P 〈0. 01, n =8). In addition, in the presence of 10^-8 mol/L salusin-[3, LVEDP (28.25±7.62 ) mmHg improved significantly compared with that in control group (52. 52±11.27 ) mmHg. CONCLUSION: These results show that salusin-β alleviates isehemia-repeffusion injury and improves heart function.
出处
《心脏杂志》
CAS
2009年第5期648-651,共4页
Chinese Heart Journal
基金
国家自然科学基金项目资助(30600763和30800375)