带鱼下脚料水解螯合物制备及其功能活性研究

Study on the functionality of protein hydrolysates of chelate by enzymolysis from Hairtail waste

通过对带鱼下脚料水解物亚铁螯合修饰及其功能性研究,为带鱼下脚料水解物的高效利用奠定理论基础。方法:采用复合酶水解法和亚铁修饰法进行初步研究。结果:未脱脂的带鱼下脚料水解螯合修饰的最适条件为蛋白质水解度为5%、螯合pH7.0、螯合温度为20℃、螯合时间为15min;经无水乙醇沉淀可获得4种螯合组分,分别为:CA,螯合后产生的沉淀物组分;CB,沉淀于50%无水乙醇中的组分;CC,悬浮于80%无水乙醇中组分;CD,沉淀于80%无水乙醇底部组分。在这4种组分中,CB抗氧化活性最高,其抗氧化活性可达到a-生育酚活性的92%;CD抗菌性最强,它对大肠杆菌、葡萄球菌沙门氏菌以及枯草芽胞杆菌都具有抗菌性。结论:带鱼下脚料水解螯合可作为食品添加剂在食品工业中应用。

This article will provide the theoretics basic by researching the functionality of protein hydrolysates of chelate by enzymolysis from Hairtail waste. Method： Complex enzymolysis and Fe^2＋-chelate methods were used in this paper. Results： The optimum formation conditions of the hydrolysate chelating Fe^2＋ are 5% of DH, pH7.0, 20 ℃ of temperature and 15 min of chelating time for FM（material not being defatted）. Four types of chelating Fe^2＋ including CA（deposit after chelating）, CB（deposit in 50% of absolute ethanol solution）, CC（suspended deposit in 80% of absolute ethanol solution） and CD（bottom deposit in 80% of absolute ethanol solution） were obtained through fractional precipitation with absolute ethanol from FM. Among all of the chelates, the CB possesses the most effective antioxidative activity and the activity is 92% as high as that of a-tocopherol; and only CD possesses the most effective antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhi and Bacillus subtilis. Conclusion： protein hydrolysates of Fe-FPH from hairtail waste can be used for food industry.