ELISA与小白鼠生物法检测腹泻性贝类毒素的研究

Diarrihetic shellfish poison detecting in scallop by ELISA and mouse biological method

以辽宁地区扇贝为原料,采用传统小白鼠生物实验法和酶联免疫吸附法对扇贝中的腹泻性贝类毒素残留量进行了检测。结果表明:扇贝样品中检测到腹泻性贝类毒素,小鼠法检测出样品中含有腹泻性贝类毒素为0.3MU/g,酶联免疫吸附法检测到样品中腹泻性贝类毒素含量为0.409μg/g。实验又将2种方法进行了比较,得出结论:2种方法结合起来作为测定腹泻性贝类毒素的方法,可以确保得到的结果更精确,更具说服力。

In this paper, Scallop in Liaoning area was used as a raw material, and mouse biological method and ELISA method were adopted to detect diarrhetic shellfish poisoning（DSP） residue in scallop. The result indicated that the scallop sample had diarrhea shellfish toxin, and the DSP content inspected by mouse biological method and ELISA method were 0.3 MU/g and 0.409 μg/g, respectively. Comparing the two methods, the conclusion could be made that the combination of them to determine DSP would be more precise and persuasive in detecting DSP.