微波辅助提取花生衣原花色素工艺优化

Microwave-assisted Extraction of Proanthocyanidins from Peanut Skin

探索花生衣原花色素微波辅助提取的最佳条件,在单因素试验的基础上,应用响应面法优化花生衣中原花色素的提取条件;研究发现料液比和萃取温度对花生衣原花色素的提取率都有显著影响,且非线性关系,最佳提取工艺参数为微波输出功率600W、乙醇体积分数40%、料液比1:24.5、提取时间91s、提取温度40℃。在此条件下原花色素的提取率为12.59%。表明响应面法对花生衣原花色素提取条件的优化是可行的,得到的原花色素提取条件具有实际应用价值。

Single factor method and response surface methodology （RSM） based on a three-level, three-variable central composite rotatable design （CCRD） were employed for the optimization of microwave-assisted extraction of proanthocyanidins from peanut skin. Results showed that ratio of material to solvent and extraction temperature both exhibited significant non- linear effects on extraction yield of proanthocyanidins; the optimum conditions for the extraction were as follows： microwave output power 600 W, ethanol concentration 40%, ratio of material to solvent 1：24.5, extraction time 91s, and extraction temperature 40℃; under the optimum extraction conditions, the extraction yield of proanthocyanidins was 12.59%. It was concluded from verification experiments that RSM is applicable to the optimization of microwave-assisted extraction of proanthocyanidins from peanut skin and the optimal extraction procedure obtained has some practical value.