摘要
以波纹巴非蛤为原料,根据最佳酶解条件制备酶解液。通过凝胶层析柱(SephadexG-25)分离水解所得粗品波纹巴非蛤酶解液,并用紫外可见分光光度计测定收集后的各管溶液,然后根据各吸光度值绘制的管数-吸光度曲线图得出第三个峰附近的管数为小分子段。根据标准曲线计算出活性肽的分子量为753.58D,最后通过反相高效液相色谱-质谱联用技术测出活性肽的分子量为679.4D。
Clam （Paphia undulata） meat was enzymolyzed and the hydolysate obtained was separated into 3 fractions by Sephadex G-25 column chromatography. The principle of gel filtration chromatography demonstrated that the last fraction eluted from Sephadex G-25 had low molecular weight, which was estimated to be 753.58 D by Sephadex G-25 column chromatography and 679.4 D by RP-HPLC-MS.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第20期143-145,共3页
Food Science
关键词
波纹巴非蛤
凝胶层析
活性肽
Paphia undulata
gel chromatography
active peptides
