罗非鱼头中硫酸软骨素提取的工艺优化

Optimization of Extraction of Chondroitin Sulfate from Tilapia Skull

目的:为罗非鱼头生产硫酸软骨素提供技术参考。方法:以罗非鱼头为原料,采用稀碱-酶解工艺,以产率和纯度为考察指标,研究了碱提取和蛋白酶水解的最佳工艺条件。结果:碱提取时碱浓度1%、时间7h、温度50℃、料液比1:5(m/V);酶解时pH7.0、加酶量1.5%、温度50℃、时间3h时有最高的产率、纯度及总得率。结论:在该条件下,产率为3.583%,纯度为81.28%,总得率为2.913%。

Chondroitin sulfate from tilapia skull was obtained using a combined method of alkaline extraction and enzymatic hydrolysis. Orthogonal array design was adopted to optimize technological parameters of alkaline extraction and enzymatic hydrolysis for maximizing yield and purity of chondroitin sulfate, respectively. The optimal procedure for preparing high-purity chondroitin sulfate from tilapia skull was based on first extraction for 7 h using 1% alkaline solution with a ratio of 1：5 （m/V） at 50 ℃ and then enzymatic hydrolysis for 3 h using compound protease with an enzyme dose of 1.5% at pH 7 and 50 ℃. Under these optimal conditions, the productivity, purity, total yield of chondroitin sulfate were 3.58%, 81.28% and 2.91%, respectively.