摘要
采用50%丙酮溶液对芡实中的多酚进行分离提取。在D101树脂对芡实多酚吸附特性研究的基础上,采用D101树脂吸附-柱层析分离法,对芡实多酚进行纯化。结果显示,D101树脂对溶液体系中芡实多酚的平衡吸附量为33.21mg/g,在蛋白质、低聚糖和多酚等组分共存的溶液体系中,D101树脂对芡实多酚的吸附具有较高的选择性。芡实多酚经D101树脂吸附后,用pH5、50%丙酮溶液(V/V)洗脱,即可将其中91.84%的芡实多酚洗脱下来。洗脱液经减压浓缩、干燥后,得到纯化芡实多酚样品,其中总酚含量达81.38%。
Crude polyphenols was isolated from gorgon nut and purified using D101-type resin adsorption-column chromatography with elution of 50% acetone aqueous （V/V） at pH 5. Results revealed that D101-type resin exhibited a high adsorption capacity （33.21 mg/g） to gorgon nut polypenols （GNPP）. High adsorption specificity to GNPP was observed in D101 resin compared with impurities in the mixture solution such as proteins and carbohydrates. Under optimal purification conditions, 91.84% GNPP could be eluted from D 101-type resin and the purity of polyphenols was 81.38 % through condensation at reduced pressure.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第20期260-264,共5页
Food Science
基金
安徽省应用化学省级重点学科建设项目(200802187C)
滁州学院自然科学研究项目(B2009kj017)
关键词
芡实多酚
吸附特性
分离
纯化
gorgon nut polyphenols （GNPP）
adsorption characteristics
isolation
purification
