顶空固相微萃取-气质联用法分析稻谷挥发性成分

Headspace Solid Phase Microextraction Coupled to GC-MS for Analyzing Volatile Components in Paddy

用顶空固相微萃取(HS-SPME)技术结合气相-质谱法(GC-MS)对不同稻谷中挥发性成分进行研究,优化顶空固相微萃取的条件,包括平衡时间、萃取时间、萃取温度以及样品量,并对不同稻谷中挥发性成分进行鉴定和分类分析。结果表明:稻谷中挥发性成分分析的最佳条件为平衡时间60min、萃取时间50min、萃取温度80℃、样品量20g。稻谷中的挥发性成分有醇类、醛类、酮类、酯类、烃类、有机酸类以及杂环类化合物等。最主要挥发性成分是醛类,其中含量最高是己醛,平均为13.31%;其次为壬醛,平均为7.93%。

Headspace solid phase rnicroextraction （HS-SPME） was employed for gas chromatography-mass spectrometric （GC-MS） analysis of volatile components in paddy in this study. The optimal HS-SPME conditions such as extraction time, temperature, sample amount and equilibration time were explored prior to quantitative investigations of volatile components in paddy, which were 50 min, 80 ℃, 20 g and 60 min, respectively. The volatile compounds in paddy consisted of alcohols, aldehydes, ketones, esters, hydrocarbons, organic acids, and heterocyclic components, etc. Aldehydes were the main volatile compounds, among which hexanal accounted for the highest percentage at 13.31% in average, followed by nonanal with an average percentage of 7.93%.