独根草不同部位多糖含量的测定

Determination of Polysaccharides in Different Parts of Oresitrophe rupifraga Bge

采用水提-醇沉法对独根草根、茎、叶中的有效成分多糖进行提取,经蒽酮-硫酸显色后,在624nm波长处比色测定。结果独根草根、茎、叶中的多糖含量分别为6.28%、10.47%、3.29%。结果表明独根草茎是独根草全株以多糖为主的重要活性部位。

Bioactive components of polysaccharides were extracted from roots, stems, and leaves of Oresitroph rupifraga Bge using water extraction-ethanol precipitation. Meanwhile, the contents of polysaccharides were determined at 624 nm by anthrone-sulfuric acid method. The contents of polysaccharides in roots, stems and leaves of Oresitrophe rupifraga Bge were 6.28%, 10.47%, and 3.29%, respectively. These results indicated that stems of Oresitrophe rupifraga Bge are the major locations of bioactive polysaccharides.