酚类化合物酶处理反应机理初探

Study on the Mechanism of Phenols Removal from Pulp and Paper Wastewater Catalyzed by Horseradish Peroxidase

研究了辣根过氧化物酶处理酚和氯酚的反应机理和动力学特征。根据酶反应动力学特征判断辣根过氧化物酶催化酚和氯酚氧化耦合反应属于乒乓双双反应机制。阐明了Ｈ２Ｏ２抑制机理，测定了酶反应中Ｈ２Ｏ２抑制的动力学参数。通过测定不同底物酶反应的动力学参数，比较了取代基数目及取代位置对酶反应的影响。

The kinetics and mechanism of the enzymatic reaction of phenol and chlorophenol were studied in this paper. It was concluded that oxidative coupling reaction catalyzed by horseradish peroxidase had the characteristic of Ping Pang reaction. The effect of H 2O 2 concentration on the enzymatic reaction was discussed in detail. The results showed that H 2O 2 had an inhibition effect on phenols removal and the inhibition mechanism of H 2O 2 was elucidated. The removal reactions for several substrates were compared according to the kinetics parameters. It was shown that there was a definite relationship between the efficiency of enzymatic reaction and the amount and position of various substituted groups for substrates.