麦洼牦牛LDH同工酶的组织特异性研究

The Peculiarity of LDH Isoenzyme in Tissues from Maiwa Yak

对麦洼牦牛心、肝、肾、肌肉及血浆ＬＤＨ同工酶谱和相对活力、ＬＤＨ总活力进行了测定．结果表明，麦洼牦牛ＬＤＨ１存在Ｆ、Ｓ两种表型．Ｆ型和Ｓ型个体之间除肾脏中ＬＤＨ５和肝脏中ＬＤＨ３相对活力差异显著外，其余组织器官及血浆ＬＤＨ同工酶相对活力、ＬＤＨ总活力无显著差异；麦洼牦牛ＬＤＨ同工酶具有明显的组织特异性，肌肉中以ＬＤＨ５和Ｍ亚基为主．其余组织器官及血浆ＬＤＨ１相对活力和Ｈ亚基比例最大；ＬＤＨ同工酶谱和相对活力具有一定的个体差异．

The LDH isoenzymes were analysed on their distribution,relative activity and total activity in heart,liver,kidney,muscle and plasma of Maiwa yak.The results showed that there existed two phenotypes in LDH 1(F,S).No siginicant differences in relative activity and total activity of LDH isoenzymes in the same tissues or plasma were observed between the F and S phenotype individual except relative activity of LDH 5 in kindey and LDH 3 in liver.The peculiarities of LDH isoenzyme in tissues were obvious,the relative activity of LDH 1 and percentage of H subunit in heart,liver,kidney and plasma were dominent while the relative activity of LDH 5 and percentage of M subunit in muscle were high.There were also some differences in LDH isoenzyme distribution and relative activity among individuals.