肿瘤坏死因子-α基因多态性与重型肝炎的相关性研究

Study on Relationship between TNF-α Gene Polymorphism and Severe Hepatitis

目的研究细胞因子TNF-α-308位点基因多态性与重型肝炎的关系。方法应用聚合酶链反应—特异性序列引物法检测60例重型肝炎患者和40例健康人的TNF-α-308位点基因型及等位基因频率,用SPSS软件分析两组人群中基因型及等位基因的分布频率。结果重型肝炎组TNF-α-308位点GG、GA、AA基因型,分别占90%、10%、0;在对照组中3种基因型,分别占95%、5%、0,两组之间的差异无统计学意义(P=0.471)。两组中等位基因G、A,分别占95%、5%和97.5%、2.5%,两组之间的差异无统计学意义(P=0.480)。结论TNF-α-308位点基因多态性可能与重型肝炎的发生无关。

Objective To investigate whether polymorphism at - 308 in the promoter region of the TNF - α gene was associated with severe hepatitis. Methods The TNF - α gene - 308 position polymorphisms was analyzed by sequence specific primers polymerase chain reaction （PCR - SSP） methods in 60 patients with severe hepatitis and 40 healthy controis. Results The frequency of genotype GG was 90.0% in the patients with hepatitis gravis, higher than the other two genotypes in the same group. The frequency of genotype GG was 95.0% which was the highest in the healthy controis there was no significant difference in genotype of TNF - α - 308 in the two groups （ P = 0.471 ）. No significant difference was found in allele frequency at TNF - α - 308 positions （ P = 0.480）. Conclusion There might be no relationship between polymorphisms of gene encoding TNF - α - 308 with severe hepatitis.