云南香格里拉地区带绦虫分子鉴定分析

Analysis and Identification of Tapeworms in Shangri-La County of Yunnan Province by Molecular Biology

目的:对云南香格里拉地区带绦虫进行分子鉴定。方法:选取成虫样本株成虫节片,抽提DNA,PCR扩增mtCOXⅠ区段,并做序列测定;结合GenBank中已知的猪带绦虫、牛带绦虫、亚洲带绦虫mtCOXⅠ序列,经DNA MAN软件处理后计算遗传距离并构建系统发育树状图。结果:XG1-3与BZ3的同源性最近,分别为98.5%,98.8%,98.0%,XG1-3与BZ2的同源性较近,而与BZ1的同源性较远,低于87%。亚洲带绦虫与牛带绦虫较接近,远离猪带绦虫。结论:云南香格里拉地区带绦虫株与亚洲带绦虫标准株相似,同属于亚洲带绦虫。mtCOXⅠ片段可用于带绦虫分类鉴定。

Objective： To identify tapeworms in Shangri-La County in Yunnan Province. Methods： Genomic DNA of taenia cestodes was isolated, and the mitochondrial cytochrome C oxydase subunit Ⅰ （mtCOX Ⅰ ） gene was amplified by polymerase chain reaction （PCR）, and then sequenced. The genomic distance and phylogenetic tree were constructed in comparison with other known mtCOX Ⅰ gene sequences of T.solium, T.saginata and T. asiatica in GenBank by using DNA MAN software. Results： The homology between XG1-3 and BZ3 is the closest, which is 98.5%, 98.8% and 98.0% ,while the relationship between T.asiatica and T.saginata is closer, and both are less relative to T.solium. Conclusion： Tapeworms from Shangri-La are similar to T.asiatica,and belong to T. asiatica; mtCOX Ⅰ fragment is suitable for identification of taenia species.